Interactive Genomic Exploration · CAG Expansion

The Genetics of Huntington's

A single letter of DNA, repeated too many times. An interactive journey through the HTT gene, the CAG triplet expansion and the inheritance of Huntington's disease.

Discover
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Causative gene (HTT)
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CAG repeats (full penetrance)
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Inheritance risk for each child
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Chromosome where HTT lies
The story at a glance

From a repeated letter to the disease

The entire journey of this page, summarized in six steps.

Starting point
DNA
Chromosome 4
HTT gene
The triplet
CAG expansion
Protein
Mutant huntingtin
Brain
Striatal damage
Outcome
Symptoms
The disease

What is Huntington's disease?

Before diving into the genetics, it helps to understand the disease that these genes explain.

Huntington's disease is a neurodegenerative, hereditary and progressive disorder that, for now, has no cure. It gradually damages deep brain structures —above all the striatum—, disrupting movement, thinking and behavior in combination.

≈ 2.7/100,000
Worldwide prevalence; around 10–14 per 100,000 in populations of European ancestry
30–50 years
Typical age of onset (mean ~40); juvenile and late-onset forms also exist
15–20 years
Average duration from the onset of motor symptoms
Autosomal dominant
Each child of an affected person has a 50% risk of inheriting it
Origin

From "hereditary chorea" to the gene

It is named after George Huntington, who in 1872 described "hereditary chorea" with remarkable clinical precision, based on families from Long Island. For more than a century it was recognized only by its symptoms; in 1993 its cause was identified —the expansion of the CAG triplet in the HTT gene—, the starting point for the rest of this page.

Symptoms

Much more than movement

Huntington's combines three broad groups of symptoms that evolve over the years:

Motor

Movement

Chorea (involuntary, "dance-like" movements), dystonia and, later on, rigidity and slowness. Problems with gait and balance, speech (dysarthria) and swallowing (dysphagia) appear.

Cognitive

Thinking

Slowing and decline of executive functions (planning, organizing, judging). Over time it evolves into a subcortical dementia; unlike other dementias, episodic memory tends to be relatively preserved in the early stages.

Psychiatric

Mood and behavior

Depression (frequent and with an elevated suicide risk), apathy, irritability, anxiety and obsessive behaviors; sometimes psychosis. They can appear years before the motor symptoms.

Clinical profiles

Not every case is the same

The most common

Classic adult form

Onset between 30 and 50 years. Chorea predominates, alongside the cognitive and psychiatric changes, with a progression of 15–20 years.

Before age 20

Juvenile form (Westphal)

Caused by very large expansions (> 60 CAG), almost always inherited from the father (an example of genetic anticipation). Instead of chorea, rigidity and slowness predominate, with seizures and academic decline.

After age 60

Late-onset form

Usually milder and more slowly progressive, with chorea as the main symptom and less cognitive involvement at the start.

Course

A disease that advances in stages

Decades can pass between inheriting the gene and the advanced stages. It is a gradual progression, not an abrupt jump.

Premanifest

The person carries the gene but has no symptoms. It can last decades.

Prodromal

Subtle signs years before diagnosis: mood changes and mild motor or cognitive alterations.

Early

Diagnosis based on motor signs. The person is still independent in daily life.

Middle

Chorea and the cognitive and psychiatric difficulties limit daily life; independence is lost.

Advanced

Total dependence, rigidity and dysphagia. Death is usually due to complications (e.g. aspiration pneumonia).

Treatment

No cure yet, but not without options

Today, treatment is symptomatic and multidisciplinary: it does not stop the disease, but it improves quality of life. Therapies that attack the cause —the gene and the protein— are reviewed at the end, once the genetics is understood.

Motor symptoms

Controlling chorea

VMAT2 inhibitors —tetrabenazine, deutetrabenazine and valbenazine (approved in 2023 for Huntington's chorea)—. Sometimes antipsychotics such as risperidone or olanzapine.

Psychiatric symptoms

Mood and behavior

SSRIs for depression and anxiety; antipsychotics for severe irritability or psychosis. Psychiatric management is key to quality of life.

Comprehensive approach

Multidisciplinary team

Physical therapy, speech therapy, occupational therapy, nutritional and psychological support, and genetic counseling for the person and their family.

Science-based educational content (George Huntington's clinical description, 1872; identification of the HTT gene, 1993; current clinical practice). It does not replace the assessment of a healthcare professional.

Foundation

The CAG triplet

DNA is read in triplets of bases. The HTT gene, on chromosome 4, contains a stretch in which the CAG triplet is repeated over and over. Each CAG codes for the amino acid glutamine.

In most people there are fewer than 27 repeats. When the stretch expands to 40 or more, the huntingtin protein carries an abnormally long tail of glutamines that misfolds, aggregates and becomes toxic to neurons.

CAGCAGCAGCAGCAGCAG··· × N repeats
Location
4p16.3
Protein
Huntingtin
Critical region
Exon 1 · CAG
Where does the gene live?

HTT, at the tip of chromosome 4

Of the 23 pairs of chromosomes, the HTT gene sits at the end of the short arm of chromosome 4, in band 4p16.3. It has 67 exons and spans about 180 kb (its historical name is IT15).

The dot marks 4p16.3, the band where HTT lies, at the tip of the short arm (p).
From gene to protein

How a repeat becomes a toxic tail

DNA is transcribed into RNA, and this is translated into protein. Each CAG adds a glutamine (Gln). If there are too many CAGs, huntingtin carries a polyglutamine tail that makes it toxic.

DNA — HTT gene
↓ transcription — messenger RNA
↓ translation — protein (huntingtin)

Interactive

The repeat that grows longer

The number of CAG repeats determines whether the disease appears and, to a large extent, when. Click each range to see what it means, or use the slider to watch the repeat grow.

20CAG repeats
Normal

In context

Huntington's is not alone

It shares its mechanism with other repeat-expansion diseases: a short stretch of DNA repeated too many times. The gene, the "letter" that is repeated and the mode of inheritance change.

DiseaseGeneRepeatThresholdInheritance
Huntington's see →HTTCAG≥ 36–40Autosomal dominant
Spinocerebellar ataxia type 1ATXN1CAG≥ 39Autosomal dominant
Myotonic dystrophy type 1DMPKCTG≥ 50Autosomal dominant
Spinal and bulbar muscular atrophy (Kennedy)ARCAG≥ 38X-linked
Fragile X syndromeFMR1CGG≥ 200X-linked
Friedreich's ataxiaFXNGAA≥ ~70Autosomal recessive

Click any row to learn why the same idea —a repeat that grows— produces such different diseases. Many share anticipation (the expansion grows across generations).

Beyond HTT

Modifier genes

The HTT gene decides who will develop Huntington's, but other genes —above all DNA-repair genes— modulate when it begins. They are the new therapeutic targets. Click a card to see the details.

Functional convergence

Gene network

HTT at the center and, around it, the genes that modulate the disease. Hover over a node to identify it; click to see the details.

A century and a half of science

Timeline of discoveries

From the first description of "hereditary chorea" to the HTT gene and the therapies that silence huntingtin.

Biology

Mechanisms of the disease

How mutant huntingtin and the continuous CAG expansion damage neurons, above all in the striatum.

Interactive · somatic expansion

The clock ticking inside your neurons

The CAG is not a fixed number: it keeps growing inside neurons with age. Move the age and switch the DNA-repair genes on or off to see how it changes.

Person's age
20years
DNA-repair genes
Switch off MSH3 —the current therapeutic idea— and watch the expansion almost come to a halt.
40
Effective CAG in the neuron at that age

Illustrative model of the trend (starting from 40 inherited CAG), not an exact clinical scale.

Why there?

Every cell has HTT, yet it is mainly the striatum that dies

Click each brain region to see why some are more vulnerable than others.

striatum
Highly vulnerable Affected later Relatively preserved
How it is measured

Biomarkers: tracking the disease without opening the brain

They make it possible to detect the disease, measure its progression and check whether a therapy is working, objectively:

Drag to see the typical course across the disease
Neurofilament light (NfL)blood · neuronal damage
Mutant huntingtinCSF · toxic protein
Striatal volumeMRI · atrophy

Illustrative values of the trend, not real clinical scales: NfL and huntingtin start to rise years before the symptoms, while the striatum atrophies progressively.

Inheritance

How is it inherited?

Huntington's is autosomal dominant: inheriting a single copy of the expanded gene from one parent is enough. Each child has a 50% chance of inheriting it.

That 50% is not an abstract statistic: for many families it is a shadow that shapes major life decisions —parenthood, work, plans for the future—. That is why the genetics of Huntington's is never separated from support and genetic counseling.

0%
Chance of inheriting it from an affected parent
0%
Penetrance with ≥ 40 repeats (if one lives long enough)
An example family — click each member
Male Female Filled = affected (carries the expansion)

The more CAG repeats, the earlier the symptoms tend to appear. In addition, the stretch can expand when transmitted —above all through the paternal line—, so the disease can start earlier in each generation (anticipation).

Anticipation

The clock that speeds up across generations

Adjust the parent's repeats and choose the transmission line. You will see the allele's tendency to grow as it passes to the children.

34parent's CAG
A child who inherits this allele: ~34–45 repeats

Illustrative model of the trend, not an individual prediction: the expansion is a random process and varies greatly from one family to another.

Typical age of onset by number of CAG repeats (approx.)

It is a statistical trend, not an exact prediction: at the same number of repeats, the age of onset varies widely between people (that is where the modifier genes come in).

Conclusions

What do we know for certain?

The essentials about the genetics of Huntington's disease:

The bottom line: for the first time in the history of this disease, knowing the gene and its mechanism is opening up therapies aimed at the cause, not just the symptoms. Genetics does not only explain Huntington's: it is pointing the way toward its treatment.

Myths

What does NOT cause Huntington's

It is a purely genetic disease. It is not brought on by lifestyle or environmental factors:

Diet Stress Injuries Infections Vaccines

General health can influence how the disease is experienced, but not its cause: that depends solely on the inherited CAG expansion.

Therapeutic approaches: where does each one stand?
Already in clinical use

They relieve the symptoms

  • VMAT2 inhibitors: tetrabenazine, deutetrabenazine and valbenazine (control chorea)
  • Antipsychotics and SSRIs for the psychiatric symptoms
  • They do not stop the disease, but they improve quality of life
In clinical trials

They attack the cause

  • Tominersen: anti-HTT antisense oligonucleotide (trials refocused on lower doses and subgroups after the phase 3 pause in 2021)
  • AMT-130: gene therapy (AAV-microRNA) under study in humans
  • Other HTT silencers in clinical development
Preclinical research

The next frontier

  • CRISPR and base editing on the expanded allele
  • Slowing somatic expansion (targets MSH3, FAN1)
  • Neuroprotection and boosting autophagy
The frontier

The latest and what's coming

Huntington's research is going through its most active moment. This is what is changing right now —and where it is headed—.

Recent advances

Discoveries that are rewriting the field

New model

The disease happens in two steps

Single-cell studies (2024–2025) indicate that the CAG expands slowly over decades within each neuron and only becomes toxic once it crosses a very high threshold (~150 repeats). It would be the somatic expansion, not the inherited allele, that sets the pace of the damage.

First signal

Gene therapy with promising data

The intracerebral gene therapy AMT-130 (AAV-microRNA) has shown in interim data a possible slowing of progression: it would be the first signal of a therapy acting on the cause. Still under evaluation.

New target

Slow the expansion, not just the gene

Establishing MSH3 and other repair genes as drivers of somatic expansion opens a different strategy: stopping the growth of the CAG before neurons reach the toxic threshold.

Future directions

Where the research is heading

Before symptoms

Treating in the premanifest phase

Relying on biomarkers such as NfL to detect early damage, the goal is to intervene years before the symptoms appear.

Selective editing

Silencing only the mutated copy

Allele-selective therapies and base editing or prime editing that correct or switch off the expanded allele while sparing the healthy copy of the gene.

Combining strategies

Attacking on several fronts

Combining HTT silencing, slowing somatic expansion and neuroprotection to multiply the effect on progression.

Research is advancing very fast and some of these results are preliminary: specific dates and data may change as the clinical trials mature.

Frequently asked questions

Common questions

The questions that come up most when learning about the genetics of Huntington's.

Can it skip a generation?
Practically no. Whoever inherits the expanded allele will develop the disease if they live long enough (very high penetrance). It may appear to "skip" a generation if a parent died young from another cause before the symptoms appeared, or in rare cases of reduced penetrance (36–39 repeats).
Can it appear with no family history?
It is uncommon, but possible. An intermediate allele (27–35) inherited from a healthy parent can expand —above all through the paternal line— and cross the pathogenic threshold in the child: this would be a new mutation. Family information may also simply be missing.
What does having 36 repeats mean? And 39?
Between 36 and 39 we speak of reduced penetrance: some people develop the disease (often late) and others do not. From ~40–42 onward, penetrance is practically complete. Below 36 it does not cause the disease.
Why do some people start earlier than others?
The number of repeats explains much of the age of onset (more repeats → earlier), but not all of it. The DNA-repair modifier genes (MSH3, FAN1…) and the somatic expansion within neurons bring the onset forward or push it back.
Can you be tested before having symptoms?
Yes, there is a predictive genetic test. It is a deeply personal decision that must always be accompanied by genetic counseling, given the absence of a cure and the strong psychological impact of the result. Many at-risk people choose not to take it.
Can passing it to your children be avoided?
There are reproductive options such as preimplantation genetic diagnosis (selecting embryos without the expansion through in vitro fertilization) or prenatal diagnosis. These are complex decisions that are addressed with specialized genetic counseling.
Sources and glossary

Where this comes from

Milestones and scientific sources on which this page is based.

Foundational milestones
1872Huntington G. On Chorea. The Medical and Surgical Reporter. The original clinical description.
1983Gusella JF et al. A polymorphic DNA marker genetically linked to Huntington's disease. Nature. Mapping to chromosome 4.
1993The Huntington's Disease Collaborative Research Group. A novel gene containing a trinucleotide repeat that is expanded and unstable on HD chromosomes. Cell. Identification of the HTT gene and the CAG expansion.
2015GeM-HD Consortium. Identification of genetic factors that modify clinical onset of Huntington's disease. Cell. DNA-repair genes as modifiers.
2016Kay C et al. Huntington disease reduced penetrance alleles occur at high frequency in the general population. Neurology. Penetrance thresholds and intermediate alleles.
Recent advances (2019–2025)
2019GeM-HD Consortium. CAG Repeat Not Polyglutamine Length Determines Timing of Huntington's Disease Onset. Cell. CAG length (not polyQ length) sets the onset: the basis of somatic expansion.
2019Tabrizi SJ et al. Targeting Huntingtin Expression in Patients with Huntington's Disease (tominersen). New England Journal of Medicine.
2020Tabrizi SJ, Flower MD, Ross CA, Wild EJ. Huntington disease: new insights into molecular pathogenesis and therapeutic opportunities. Nature Reviews Neurology. Landmark review.
2023Furr Stimming E et al. Valbenazine for chorea associated with Huntington's disease (KINECT-HD): phase 3 trial. The Lancet Neurology. Basis of its approval.
2024–25uniQure. AMT-130 (AAV5-miHTT gene therapy): interim phase I/II results suggesting a slowing of progression (company announcements and conferences; pending peer-reviewed publication).
2025Handsaker RE et al. Long somatic DNA-repeat expansion drives neurodegeneration in Huntington's disease. Cell. The "two-step" model of the disease.
2017+Byrne LM, Wild EJ et al. Neurofilament light (NfL) in blood as a biomarker of progression. The Lancet Neurology and subsequent work.
Databases and outreach
DatabasesOMIM #143100, ClinGen, GWAS Catalog, Enroll-HD and HDBuzz (peer-reviewed science outreach).

An educational synthesis page; not a primary clinical source. Some 2024–2025 results are preliminary and may change. For medical decisions, consult professionals and the official resources of Huntington's disease associations.

Glossary

Key terms

AlleleEach of the two copies of a gene (one from each parent).
In Huntington's it is enough for one of the two copies of the HTT gene to carry the CAG expansion to develop the disease —that is why it is dominant—. The healthy copy keeps making normal huntingtin, but it does not prevent the damage.
Autosomal dominantA single mutated copy is enough to develop the disease; each child has a 50% risk.
Autosomal = the gene is on a non-sex chromosome (chromosome 4), so it affects men and women equally. Dominant = a single mutated copy is enough. Hence the 50% chance for each child of an affected person.
ExonThe part of a gene that does code for protein. The CAG expansion is in exon 1 of HTT.
Genes alternate exons (translated into protein) and introns (spliced out). Because the CAG repeat lies in exon 1, it ends up translated into the protein as a tail of glutamines.
PenetranceThe probability that a carrier of the mutation will go on to manifest the disease.
From ~42 CAG onward it is practically complete (the person will develop the disease if they live long enough). Between 36 and 39 it is "reduced": some people develop the disease and others do not. That is why 40–41 are considered very high penetrance, but not 100% guaranteed.
AnticipationThe repeat's tendency to grow across generations, bringing the onset forward.
It occurs above all through the paternal line: as sperm form, the CAG tends to expand further. That is why the juvenile form is almost always inherited from the father.
Somatic expansionGrowth of the CAG within the neurons themselves over the course of life.
Somatic = in the body's cells (not in eggs or sperm). The CAG keeps growing inside the striatal neurons for years; once it crosses a certain threshold it becomes toxic. It is the driver of progression and the main current therapeutic target.
MMRThe DNA mismatch repair system (MSH3, MLH1…); it modulates the expansion.
It normally corrects DNA errors, but when faced with the CAG repeat it "slips" and, paradoxically, lengthens it. Slowing MMR components (above all MSH3) is today one of the major therapeutic strategies.
Polyglutamine (polyQ)The chain of glutamines produced by the CAG repeat; if very long, it makes huntingtin toxic.
Each CAG codes for a glutamine (Gln). Too many CAGs give an abnormally long tail of glutamines that misfolds, aggregates and becomes toxic. This is the mechanism that Huntington's shares with other "polyQ diseases", such as several ataxias (SCA).
Test what you've learned

Interactive quiz

Six questions to check what you take away. It grades itself: click an answer and you'll instantly see whether you got it right, with the explanation.

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